Web(F and G) Transwell assay was employed to determine cell migration and invasion (100×) (ANOVA). (H and I) Lactate production or glucose consumption was measured by lactate assay kit or glucose assay kit, respectively (ANOVA). … WebFeb 14, 2024 · Flow cytometry assay indicated that zonisamide abolished microglial reactive oxygen species (ROS) generation in PD models. Extracellular flux assays showed that LPS exposure to BV2 cells at 1 μg/mL drastically reduced the mitochondrial oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
XFe-24 Extracellular Flux Analyzer (Seahorse Bioscience)
WebThe XFe-24 flux analyzer measures real time kinetic of the media flux in live cellular environment to determine in vitro oxygen consumption rate (OCR), and extracellular acidification rate (ECAR), in order to assess cellular metabolic functions such as oxidative phosphorylation, glycolysis, and fatty acid oxidation. XFe-24 assays apply to all fields of … WebDec 9, 2024 · Cells were washed and cultured in a 37°C incubator without CO 2 for 1 h prior to the assay. For ECAR measurement, stock compounds from the XF Glycolysis Stress Test Kit were diluted into the XF Glycolysis Stress Test assay medium and loaded into the cartridge ports to achieve final concentrations of 10 mM glucose, 2 μM oligomycin, and … hoyt models by year
Glycolysis Assay [Extracellular acidification] (ab197244)
WebSep 20, 2024 · In simple terms, the EICAR test file is a computer file that was developed to test the response of antivirus (anti-malware) products. It is not a real computer virus, but it mimics malware, and thus allows for safe … WebSpecifically, this machine measures glycolysis by analyzing the extracellular acidification rate (ECAR) and measures mitochondrial oxidative phosphorylation on the basis of the … WebAnother alternative to Seahorse extracellular flux technology is the Clark Oxygen electrode for oxygen uptake rates. For glycolysis - you can use an enzyme-linked assay whereby you measure the ... hoyt museum new castle pa