WebSep 23, 2024 · C. glutamicum is a non-pathogenic, aerobic, Gram-positive, biotin-auxotrophic soil bacterium that was initially described to be a natural producer of L-glutamate (Kinoshita et al., 1957).Since its discovery, the most appreciated application for this organism is L-glutamate and L-lysine production (Eggeling and Bott, 2015), however, … WebFeb 23, 2024 · E. coli DH5α was used for the construction of plasmids. C. glutamicum J0 was used as the starting strain for engineering to overproduce 5-ALA. For plasmid construction, E. coli DH5α was cultured in Luria-Bertani broth (10 g/L tryptone, 10 g/L NaCl, and 5 g/L yeast extract) or on LB agar plates (2% w / v agar) at 37 °C and 220 rpm.
Growth-rate dependency of ribosome abundance and …
WebSep 4, 2003 · Corynebacterium glutamicum is an aerobic bacterium that requires oxygen as exogenous electron acceptor for respiration. Recent molecular and biochemical analyses together with information obtained from the genome sequence showed that C. glutamicum possesses a branched electron transport chain to oxygen with some remarkable features. Webfrom a dam)–dcm) E. coli strain or synthetic DNA transformed C. glutamicum with an e†ciency of up to 106 cfu lg)1 DNA (Vertes et al. 1993; Ankri et al. 1996a). The electrotransformation e†ciency of some C. glutamicum strains can also be increased by extracting the plasmid DNA from a starved E. coli host (Ankri et al. 1996b). marcatori uefa champion league
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WebDec 1, 2024 · Overall, this study resulted in the identification of causative key mutations improving the growth of C. glutamicum on glucose. These identified mutational hot … WebOct 4, 2024 · Regulated single-cell growth is crucial for the survival of a bacterial population. At the population level, fundamental laws of growth were discussed as early as the beginning of the 20th century, and distinct population growth phases were identified and attributed to bacterial growth (Lane-Claypon, 1909; Buchanan, 1918; Monod, 1949).At … WebDec 1, 2024 · Isolated top clones (UBw and UBm) showed an about 26% increased growth rate on glucose minimal medium. Genome sequencing of evolved clones and populations resulted in the identification of key... marcato set